Substrate thickness |
0.17mm = 170μm |
Substrate material |
Glass, compatible with immunofluorescence and confocal microscopy |
Number of channels per biochip |
8 |
LOW FLOW RATE BIOCHIPS: |
|
Channel dimensions |
160μm (H) x 1600μm (W) x 28mm ≡ 0.16mm (H) x 1.6mm (W) x 28mm |
Volume of each channel
|
7.168μL |
Volume of protein required for coating |
~20μL |
HIGH FLOW RATE BIOCHIPS: |
|
Channel dimensions
|
80μm (H) x 800μm (W) x 28mm ≡ 0.08mm (H) x 0.8mm (W) x 28mm |
Volume of each channel
|
1.79μL |
Volume of protein required for coating |
10μL |
|
|
Volume of reservoir to hold sample |
0.5mL; also possible to connect via tubing to eppendorf if greater sample volume or high shear stress / flow rate assay is being performed |
|
|
Thrombosis assays - recommendations for whole blood: |
- Biochips: Vena8 with Glass Coverslips Adhered, Treated with Cu2+ histidine tag; Low Flow Rates; Pack 10 or Vena8 with Glass Coverslips Adhered, Treated with Cu2+ histidine tag; High Flow Rates; Pack 10
- Pumps: ExiGo or Mirus Evo Nanopump
- Low flow rates / shear stresses: 0.45 - 9 dyne/cm2
- High flow rates / shear stresses: 90 - 180 dyne/cm2
|
Stem cell assays - recommendations for stem cell culture: |
- Biochips: Vena8 with Glass Coverslips Adhered, Non-treated; Low Flow Rates; Pack 10
- Pumps: Kima pump
- Low flow rates / shear stresses: 1 dyne/cm2
|
Biofilm assays - recommendations for biofilm culture: |
- Biochips: Vena8 with adhesive bottom layer; Low Flow Rates; Pack 10; biochips have a protective cover which can be peeled away to reveal adhesive bottom layer to which any material may be attached by the user.
- Pumps: Kima pump
- Low flow rates / shear stresses: 1 dyne/cm2; particularly suited for biofilm applications with Kima pump achieving shear stresses of 1dyne/cm2 where different substrate materials may be easily tested.
|