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 Cellix >> 生物芯片 >> 爱尔兰Cellix公司生物芯片
 
  
产品编号:
3159553616
产品名称:
爱尔兰Cellix公司生物芯片
产品品牌:
Cellix
产品备注:
产品类别:
Cellix
 
       产 品 说 明

爱尔兰Cellix代理,爱尔兰Cellix销售。

VENA8 FLUORO+

Specifications

 Substrate thickness  0.17mm = 170μm
 Substrate material  High quality plastic, compatible with immunofluorescence and confocal microscopy
 Number of channels per biochip  8 
 Height of channels  0.1mm = 100μm
 Width of channels  0.4mm = 400μm
 Length of channels  28mm = 2.8cm
 Volume of each channel  0.8μL
 Volume of protein required for coating  10μL
 Volume of reservoir to hold sample  0.1mL; also possible to connect via tubing to eppendorf if greater sample volume or high shear stress / flow rate assay is being performed

VENA8 ENDOTHELIAL+

Specifications

 Substrate thickness  0.5mm = 500μm
 Substrate material  High quality plastic, compatible with brightfield, phase contrast imaging and fluorescence microscopy
 Number of channels per biochip  8 
 Height of channels  0.12mm = 120μm
 Width of channels  0.8mm = 800μm
 Length of channels  28mm = 2.8cm
 Volume of each channel  2.69μL
 Volume of protein required for coating  12μL (e.g. fibronectin to promote adhesion of cells for culture)
 Volume of cell sample required for seeding  5μL; cell concentration = 15 x 10cells/mL
 Growth area per channel  0.096 mm2
 Typical number of HUVECs cultured per channel   ~ 40,000 cells per channel
 Typical number of cells retrieved from one chip  ~ 320,000 cells per chip 
 Volume of reservoir to hold sample  0.5mL; also possible to connect via tubing to eppendorf if greater sample volume or high shear stress / flow rate assay is being performed

VENAT4 - CHEMOTAXIS, TRANSMIGRATION & INVASION ASSAYS

Specifications

 Substrate thickness  0.5mm = 500μm
 Substrate material  High quality plastic, compatible with brightfield, phase contrast imaging and fluorescence microscopy
 Number of channels / microwells per biochip  4
 Height of channels  0.1mm = 100μm
 Width of channels  0.8mm = 800μm
 Length of channels  28mm = 2.8cm
 Volume of each channel  2.253μL
 Volume of protein required for coating channel  50μL
 Volume of gel required for microwell  30μL
 Volume of reservoir to hold sample  0.5mL; also possible to connect via tubing to eppendorf if greater sample volume or high shear stress / flow rate assay is being performed

VENA8 WITH GLASS COVERSLIP (ADHERED / NOT ADHERED; TREATED / NOT TREATED)

Specifications

Substrate thickness  0.17mm = 170μm
Substrate material  Glass, compatible with immunofluorescence and confocal microscopy
Number of channels per biochip  8 
LOW FLOW RATE BIOCHIPS:  
Channel dimensions  160μm (H) x 1600μm (W) x 28mm ≡  0.16mm (H) x 1.6mm (W) x 28mm
Volume of each channel
 7.168μL
Volume of protein required for coating  ~20μL
HIGH FLOW RATE BIOCHIPS:  
Channel dimensions
 80μm (H) x 800μm (W) x 28mm ≡  0.08mm (H) x 0.8mm (W) x 28mm
Volume of each channel
 1.79μL
Volume of protein required for coating  10μL
   
Volume of reservoir to hold sample  0.5mL; also possible to connect via tubing to eppendorf if greater sample volume or high shear stress / flow rate assay is being performed
   
Thrombosis assays - recommendations for whole blood:
  • Biochips: Vena8 with Glass Coverslips Adhered, Treated with Cu2+ histidine tag; Low Flow Rates; Pack 10 or Vena8 with Glass Coverslips Adhered, Treated with Cu2+ histidine tag; High Flow Rates; Pack 10
  • Pumps:  ExiGo or Mirus Evo Nanopump
  • Low flow rates / shear stresses:  0.45 - 9 dyne/cm2
  • High flow rates / shear stresses:  90 - 180 dyne/cm2
Stem cell assays - recommendations for stem cell culture:
  • Biochips: Vena8 with Glass Coverslips Adhered, Non-treated; Low Flow Rates; Pack 10
  • Pumps:  Kima pump
  • Low flow rates / shear stresses:  1 dyne/cm2
Biofilm assays - recommendations for biofilm culture:
  • Biochips: Vena8 with adhesive bottom layer; Low Flow Rates; Pack 10; biochips have a protective cover which can be peeled away to reveal adhesive bottom layer to which any material may be attached by the user.
  • Pumps:  Kima pump
  • Low flow rates / shear stresses:  1 dyne/cm2; particularly suited for biofilm applications with Kima pump achieving shear stresses of 1dyne/cm2 where different substrate materials may be easily tested. 

VENADELTAY1 AND VENADELTAY2

Specifications

 Substrate thickness  0.5mm = 500μm
 Substrate material  High quality plastic, compatible with brightfield, phase contrast imaging and fluorescence microscopy
 Number of channels per biochip  4
 Height of channels  0.1mm - 0.12mm = 100μm - 120μm
 Width of channels  0.4mm - 0.8mm = 400μm - 800μm
 Length of channels  28mm = 2.8cm
 Volume of each channel  0.8μL -  2.69μL
 Volume of protein required for coating  10μL - 12μL
 Volume of reservoir to hold sample  0.1mL; also possible to connect via tubing to eppendorf if greater sample volume or high shear stress / flow rate assay is being performed

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